Dans’ Feed Artemia Enrichment

dans-feed

HOW IT CAME ABOUT!
Dans’ Feed is a select combination of enrichments blended together in proprietary amounts for the enrichment of Artemia. It is also often used for Rotifers as well. At one time we used several different enrichments, as we weren’t completely happy with any single enrichment. We took a hard look at how were doing the enrichments and felt there had to be a better way. We were adding several different products in small amounts, it was very difficult to be precise in the measurements. This in turn can result in mixed results with rearing seahorses depending upon who did the enriching and how close to being consistent they were. So we did some research and then spent many months experimenting with different formulations of various enrichments and amounts in an effort to come up with a standardized recipe. After each blend of enrichments was used up, we would compare notes to previous batches and make small adjustments. Finally, many months later, we settled on a recipe that showed the best results for us. This proved to be much more consistent and predictable with the results with our seahorse fry.

Our original intent was to make this for in house use but one day a customer asked us what we used to enrich our Artemia. When we explained that we had our own recipe, the customer asked if could he buy some. We sold him some and he told some other folks what he was using and how happy he was with it. In a short period of time we had several customers who bought some as well. Word of mouth has spread to where our current sales of the product are.

WHAT IS IN DANS’ FEED?
Dans’ Feed is a blend of several different enrichments; AlgaMac 3050, AlgaMac Protein Plus, Spirulina, Astaxanthin, Ascorbyl Palmitate and L-ascorbyl-2-polyphosphate (AsPP)

There is also an Immune Boosting Formula with Beta Glucan and Allisure AC-23 added.

Unlike emulsified oil based products, it has a very long shelf life. Stored properly, it can last for up to 24 months.

The first and largest ingredient is AlgaMac 3050, manufactured by Aquafauna Bio-Marine Inc. A pure schizochytrium algae, drum dried and in flake form. It is designed as a direct algae replacement or substitute, used directly as an enrichment, direct feed or formula ingredient. It is very high in the Omega-3 fatty acids with the fat content at 56.2% with 43.27% of those being DHA (22:6w3). The DHA to EPA (20:5w3) is about 15 to 1. It is also rich in vitamins Biotin, Choline, Folic Acid, Inositol, Niacin, Panthothenic Acid, Pyridozine, Riboflavin, Thiamine, Vitamin A, Vitamin B12, Vitamin C, Vitamin D and Vitamin E and the Amino Acids Alanine, Arginine-HCL, Aspartic Acid, Glutamic Acid, Glycine, Histidine-HCL, Isoleucine, Leucine, Lysine-HCL, Phenylalanine, Proline, Serine, Threonine, Tyrosine, and Valine. For a complete nutritional profile go to http://www.aquafauna.com/Profiles-AlgaMac-3050.htm.

The next ingredient is AlgaMac Protein Plus. Designed as a larval diet it contains heterotrophic and phototrophically produced algae, fungi and yeast cells. As the name implies, it is higher in protein with 42.9%. However the Omega-3 fatty acids contains a 95 to 1 DHA to EPA ratio.

Next it contains Spirulina Powder which is a type of blue green micro algae that is rich in protein, vitamins, minerals, carotenoids and antioxidants. This product, used widely in aquaculture can promote growth, increase appetite, disease resistance and survival rates and can enhance color.

Astaxanthin is also added to help boost color.

Ascorbyl Palmitate is a fat soluble form of Vitamin C. Artemia convert this back to Ascorbic Acid (Vitamin C).

L-ascorbyl-2-polyphosphate (AsPP) a stabilized form of Vitamin C.

Immune Boosting Formula also contains

Beta Glucan is an immunostimulant.

Allisure AC-23 is a stabilized form of Allicin, the primary ingredient in garlic responsible for increased immunostimulation, Also has antibacterial, antiparasitic and antifungal properties.

DIRECTIONS ON HOW TO USE DANS’ FEED
For Artemia nauplii

After hatching Artemia cysts, rinse the nauplii well and move to a clean enrichment container with clean new saltwater and heavy aeration. Prepare the Dans’ Feed, added at a rate of 0.2 grams (approximately 1/8 tsp) per liter, by blending well in water. A stick blender is ideal for this process as you don’t have to use as much water and clean up is easier and faster. Once blended, the enrichment is added to the enrichment container.

Enriching should be done for a minimum of 12 hours. It takes approximately 12 hours for the gut of Artemia nauplii to be fully packed. For best results, enrich the nauplii for a total of 24 hours as they will assimilate much of the nutrients from the first 12 hours and re-pack the gut. They will be almost twice as nutritious as those enriched for only 12 hours. To do this, take down the enrichment container after 12 hours, rinse the nauplii well and place in another clean enrichment container and add the enrichment as done originally.

It is suggested to use freshwater when blending the enrichment but many find they well using saltwater.

If you encounter a lot of foam during the blending process, you can pour the blended enrichment through a clean rotifer sieve to remove the foam.

Once enrichment is complete, rinse the nauplii and feed out. If there is leftover nauplii, you place it in the refrigerator for up to 24 hours. The Artemia nauplii activity will slow down and it will conserve their nutritional value. Once added to tank water, they will begin to buzz around again within 5 minutes after they warm up.

You should not enrich with a high fatty acid enrichment for more than 24 hours. So for growing out Artemia nauplii, another product lower in fatty acids should be used. The nauplii don’t digest much of the fatty acids so it builds up in their gut and eventually clogs them up to where they will perish. A live micro algae or spirulina works well for growing them out. Enriching with a high fatty acid enrichment should only be done before feeding out the Artemia.

Enriching adult Artemia
This is done very similar to the nauplii. The difference is that usually don’t have to have as strong aeration and the enrichment periods are much shorter. Adult Artemia typically pack the gut within 2 hours and by 4 hours they have assimilated and re-packed the gut. Otherwise the process is the same.

Enriching Rotifers
Harvest the amount of Rotifers needed for feeding. Rinse with clean new saltwater and place in a clean enrichment vessel with clean new saltwater and slow aeration. Measure Dans’ Feed at a rate of 0.2 grams (approximately ½ tsp) per liter of enrichment vessel and blend for 2 minutes. Add to enrichment vessel for 4 hours. Harvest and feed out.

STORAGE
Dans’ Feed should be good for 2 years provided it is stored in sealed container in the refrigerator or freezer.

WHERE TO PURCHASE
In the US, it can be purchased directly from us. Listed under foods, enrichments. In the UK, it can be purchased from ZMSytems (http://www.zmsystems.co.uk/index.php?app=gbu0&ns=prodshow&ref=danbs)

FURTHER READING

Browne, Robert A., Sorgeloos, Patrick and a Trotman, Clive N. 1991. Artemia Biology. CRC Press, Inc.

Al-Ansari, Adly. 2007. Technical Manual for Live Food Production. Public Commission for the Proctection of Marine Resources, Environment, and Wild Life, Directorate of Marine Resources, Kingdom of Bahrain.

Hoff, Frank H. and Snell, Terry W. 2007. Plankton Culture Manual. Florida Aqua Farms Inc.

Lavens, Patrick and Sorgeloos Patrick. 1996. Manual on the Production and Use of Live Food for Aquaculture. Laboratory of Aquaculture and Artemia Reference Center, University of Ghent, Ghent, Belgium, FAO Fisheries Technical Paper 361.

Strottrup, Josianne G. and McEvoy, Leslie A. 2003. Live Feeds in Marine Aquaculture. Blackwell Science.

The Effects of Different Feed Enrichments on Survivorship and Growth of Early Juvenile Longsnout Seahorse, Hippocampus reidi. Nancy Pham and Junda Lin, World Aquaculture Society, Volume 44, Issue 3, Pages 435-446. 2013